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KMID : 0880220100480020223
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Journal of Microbiology
2010 Volume.48 No. 2 p.223 ~ p.228
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Protection Against Helicobacter pylori Infection by a Trivalent Fusion Vaccine Based on a Fragment of Urease B-UreB414
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Li Wang Wang
Liu Xiao-Fei
Shi Yun
Yuan Xiao-Peng
Mao Xu-Hu
Wu Chao
Zhang Wei-Jun
Liu Kai-Yun
Guo Gang
Lu Dong-Shui
Tong Wen-De
Wen Ai-Dong
Zou Quan-Ming
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Abstract
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A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.
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KEYWORD
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H. pylori, multivalent vaccine, urease B subunit, H. pylori adhesin A, heat shock protein A
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